The absorption spectrum from the Nanodrop™ spectrophotometer indicated that the Naupaka (S. taccada) juice does contain UV absorbing properties that can be exploited, with a calculated SPF of 20.
The plates exposed to UV radiation (253 nm) showed no signs of viability with the conditions given by the experiments. Therefore, the results do not support the original hypothesis.
These results suggest that we need to critically review our protocols and methods before we repeat the experiment.
In future experiments, S. cerevisiae will be exposed to UVB and UVA rather than UVC (germicidal). The distance from the UV source will be subject to variations.
The toxicity of Naupaka Kahakai extracts will also be tested on coral colonies in lab conditions in hopes of making a reef safe sunblock.
Ackowledgements: A big Mahalo to Professor Mike Ross, MSc. and John Berestecky, Ph.D, for mentoring and advising my research project. Thank you to Colleen Allen, Brent Shigano, Draven Aquino and Kara Riggsbee for supervising and helping me in my lab experiments.